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General Information
Rat: Sprague Dawley
The full-length rat Parkin gene is expressed unilaterally in the SN using the Adeno-Associated-virus 2/6 (AAV 2/6).
Endogenous rat Parkin: yes
Corresponding human genotype: Parkin was the first gene associated with autosomal recessive Parkinson’s disease (PD).
Targeted gene: Parkin
References: PMID Liu-2013-23313192
Viral injection
Injection coordinates: Left SN -. one deposition site : AP: -5.6 mm, ML: -2 mm, DV: -7.6 mm.
Volume of injection and viral titer: Two microliters of virus-containing suspensions of 1×107 or 2×107 TUs are microinjected into the brain
Transgene expression
3 weeks post-injection: The transgene is expressed in the SN and striatum (3-8 fold increase compared to the non-injected side)
Neurodegeneration
3 weeks post-injection: None to very mild decrease in striatal density of TH- positive fibres is observed in the injected side.
Dopamine Homeostasis
Not reported
Inclusions
Not reported
Motor Behaviours
Not reported
Response to dopaminergic treatment
Not reported
Non-motor Behaviours
Not reported
Electrophysiology
Not reported
Neuroinflammation
Not reported