BAC-huLRRK2-WT-Rat

General Information

Rat: Sprague-Dawley

The full-length human wild type LRRK2 was introduced in Sprague-Dawley rats using a bacterial artificial chromosome (BAC).

Endogenous rat LRRK2 : yes

Corresponding human genotype: LRRK2 is the greatest known genetic contributor to Parkinson’s disease.

Mutated gene: LRRK2

References: Sloan 2016

Transgene expression

• The transgene is expressed throughout the brain at different levels (4-5 fold higher than the endogenous LRRK2). The human LRRK2 protein is present at low level in the SNand high level in the hippocampus.

Neurodegeneration

• Not reported

Dopamine Homeostasis

• Not reported

Inclusions

• 18-21 months: No inclusions positive for alpha-synuclein, phosphorylated Tau (pSer202/Thr205: AT8) or ubiquitin observe in the SNpc.

Motor Behaviours

• 3-6 and 18-21 months: Motor performances of the transgenic animals appear normal on the accelerating rotarod, grip test and drag test.

Response to dopaminergic treatment

• Not applicable in the absence of motor impairment.

Non-motor Behaviours

• 3-6 and 18-21 months: Performances of the transgenic animals on the spontaneous alternation test appear normal.

Electrophysiology

• 6 and 12 months: no changes in electrical stimulation-evoked DA are observed in the dorsal or ventral striatum. Dopamine content and reuptake rate are not altered.
• 18-21 months: a decrease in electrical stimulation-evoked DA release is observed in the dorsal striatum. DA content and reuptake rate are not altered suggesting a reduced ability to release DA. No difference is observed in the firing rates of SN dopamine neurons.

Neuroinflammation

• 18-21 months: no changes in the number of glial cells (microglia) are observed compared to non-transgenic littermates.