G51D SNCA rat (MRC Centre for Regenerative Medicine)

http://www.crm.ed.ac.uk/people/tilo-kunath
[email protected]

United Kingdom

CRISPR/Cas9 technology was used by Dr Tomoji Mashimo (Kyoto Univeristy) to introduce a single amino acid mutation (Gly-51-Asp) into the endogenous rat alpha-synuclein gene. This rat model is currently being characterised, but preliminary transcriptomic data on the midbrain of G51D/+ 11-month-old animal shows reduced expression of dopaminergic transcripts, including tyrosine hydroxylase. Frozen sperm for this model have been deposited into the National BioResource Project for the Rat in Japan ((http://www.anim.med.kyoto-u.ac.jp/NBR).
Parkinson’s disease & PD-related disorders
Animals| Frozen sperm
Yes
Access independent of collaboration with PI| International access
No
Genetically modified

Yes
PD
Rat
Yes
characterisation in-progress
reduced tyrosine hydroxylase expression in midbrain of G51D/+ rats
G51D/+ and G51D/G51D

National BioResource Project for the Rat in Japan
Yes
2019
Indefinite
No

Experimental Models
United Kingdom

2016