Principal Investigators

    Ing. Jaroslav Šebestík Ph.D.


    Institute of Organic Chemistry and Biochemistry AS CR, v.v.i.

    Contact information of lead PI


    Czech Republic

    Title of project or programme

    3-Nitro-L-tyrosine in structures of neurodegenerative proteins

    Source of funding information

    Czech Science Foundation

    Total sum awarded (Euro)

    € 184,630

    Start date of award


    Total duration of award in years



    Research Abstract

    The influence of oxidative stress in protein metabolism on the structure of neurodegenerative proteins will be investigated on chemical models
    by chiroptical spectroscopies combined with theoretical calculation of spectra. One of the results of oxidative stress is mutation of tyrosine to
    3-nitro-L-tyrosine in proteins. This mutation change significantly the hydrophobicity and acidity of the residue. Since the secondary structure of
    mutated proteins could be altered, the mutation could influence the onset of the neurodegenerative diseases caused by changes of the
    protein conformation. Selected targets for such conformational changes will be the prion protein and alpha-synuclein models. The first one
    plays role in transmissible spongiform encephalopathies and the second one in Parkinson’s disease. The spectroscopic data will be correlated
    with the ability of nitrated protein to aggregate and to form amyloidogenic fibrils. The spectroscopic measurement of sparingly soluble
    amyloidogenic proteins will be allowed by signal enhancement on the surface of silver and gold nanoparticles.

    Further information available at:

Types: Investments < €500k
Member States: Czech Republic
Diseases: N/A
Years: 2016
Database Categories: N/A
Database Tags: N/A

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