Name of Fellow

    Dr James Sleigh



    Wellcome Trust

    Contact information of fellow


    United Kingdom

    Title of project/programme

    Dissecting Neuronal Specificity in Hereditary Neuropathy.

    Source of funding information

    Wellcome Trust

    Total sum awarded (Euro)

    € 338,950

    Start date of award


    Total duration of award in years


    The project/programme is most relevant to:

    Neurodegenerative disease in general


    Motor Neuron | Neurodegen

    Research Abstract

    Using fruit fly and cellular models, we have already established non-cell autonomous toxic effects from muscle as the process by which GARS mutations cause neuronal-specific pathology, and provided a potential mechanism by which this might be mediated. This led to my working hypothesis that disruption of axonal guidance through direct disturbance of axonal transport may be the defective downstream process leading to neuropathology. In order to test my hypothesis, I now propose to extend my work into mammals by developing mouse and human neuronal models of CMT2D and to combine these with live-imaging studies. I will characterise both cell autonomous and non-cell autonomous defects of primary neuronal cultures from Gars mice and induced pluripotent stem cell-derived motor neurons from CMT2D patients. I will also monitor the dynamics of axonal transport and somatic sorting of signaling endosomes and other organelles (e.g. mitochondria) in my cultures, and subsequently confirm and expand t hese results in vivo by monitoring axonal transport by intravital microscopy in Gars mice. I believe that this combined approach will provide a powerful platform to deduce the disease mechanism linking toxic mutations in a housekeeping gene to the selective neuropathy observed in CMT2D patients.

Types: Fellowships
Member States: United Kingdom
Diseases: Neurodegenerative disease in general
Years: 2016
Database Categories: N/A
Database Tags: N/A

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