Principal Investigators

    Truant, Ray


    McMaster University

    Contact information of lead PI



    Title of project or programme

    Elucidating the Protective Mechanism of Phospho-N17 Huntingtin in Huntington's disease

    Source of funding information


    Total sum awarded (Euro)

    € 581,466

    Start date of award


    Total duration of award in years


    The project/programme is most relevant to:

    Huntington's disease


    Research Abstract

    Huntington’s disease (HD) is one of a family of neurodegenerative diseases that occurs in mid-life that affects 1 in 6000. The exact cause of Huntington’s disease is a mutation in the gene that codes for the huntingtin protein. From the last 5 years of CIHR funding, we have discovered important new functions for the huntingtin protein mediated by a small region of 17 amino acids (N17). N17 can be chemically modified in brain cells by kinase enzymes. This modification affects two amino acids that are located near the HD mutation. Based on our work, others have shown that if we can keep N17 modified, HD does not develop in the mouse. In 2011, we made two important new discoveries: that this critical modification can be prevented or increased by drugs called kinase inhibitors; and that huntingtin has an important role at the cell cytoskeleton during cell stress. We have discovered that mutant huntingtin is not properly modified in N17, but that drugs can restore this. These drugs are under development for other diseases by pharma, which has led to new collaborations. In the next 5 years, we will use sophisticated methods of microscopy that analyse properties of light behaviour in living cells, or Biophotonics. From these methods, we can watch in real time in live cells how the drugs we have identified can restore the mutant protein to a less toxic form. Our goals are to extend this technology to measure the shape of huntingtin protein from cells derived from HD patents, and eventually create an assay that can be used on patient blood samples. This assay can be used as a marker of disease progress, or to tell us if drugs are working in patients. There are tremendous advantages to studying neurodegeneration in Huntington’s disease, as there is a single defined cause, and this disease could be used to model drug discovery in more complex neurodegenerative diseases such as Alzheimer’s disease.

    Lay Summary

    Further information available at:

Types: Investments > €500k
Member States: Canada
Diseases: Huntington's disease
Years: 2016
Database Categories: N/A
Database Tags: N/A

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