Principal Investigators

    MUDr. Vladimír Doležal, DrSc.


    Institute of Physiology of the AS CR, v.v.i.

    Contact information of lead PI


    Czech Republic

    Title of project or programme

    Search of mechanisms responsible for adverse effects of amyloid beta on muscarinic receptor transmission

    Source of funding information

    Czech Science Foundation

    Total sum awarded (Euro)

    € 166,555

    Start date of award


    Total duration of award in years



    Research Abstract

    Regular finding at autopsy in Alzheimer´s disease is loss of basal forebrain cholinergic neurons and damage of cerebral cortex cholinergic markers. In concert, the only approved drugs with limited benefit are cholinesterase inhibitors. We found in transgenic APPswe/PS1dE9 mouse model of the disease the early weakening of muscarinic signaling preceding amyloid deposition. It was not due to changes in muscarinic receptors or G-proteins expression, pointing to damage of receptor/G-protein interaction within membrane. The major obstacle in devising efficient therapy is a poor knowledge of likely reversible initial changes during the asymptomatic phase of the disease. The objective of this project is to identify mechanisms of initial muscarinic impairment by beta-amyloid. Knowledge of molecular mechanisms and time-course is of prime importance for development of new drugs and therapeutic strategies enabling proper selection of drug targets that are likely to depend on the stage of impairment but may not necessarily be confined to agents directly acting on muscarinic receptors.

    Further information available at:

Types: Investments < €500k
Member States: Czech Republic
Diseases: N/A
Years: 2016
Database Categories: N/A
Database Tags: N/A

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