General Information

Mice: C57/BL6

 Expression the human wild type PARKIN gene unilaterally in the SN using recombinant Adenovirus-Associated virus 2/8 (AAV 2/8).

Endogenous Parkin: Yes

Corresponding human genotype: Parkin was the first gene associated with autosomal recessive Parkinson’s disease (PD).

Targeted gene: Parkin

References: Van Rompuy 2014

Viral injection

Injection coordinates: right SN -. one deposition site: AP −3.1 mm, ML −1.2 mm, DV −4.0 mm.

Volume of injection and viral titer: Two microliters of rAAV vector is injected at a concentration of 2 × 10¹¹ GC/ml

Transgene expression

The level of transgene expression is unknown, however, transduction efficacy of the nigral dopaminergic cells reaches 90%.

Neurodegeneration

12 months: No visible degeneration can be observed between the mice injected with the recombinant viral vectors containing the human Parkin or GFP.

Dopamine Homeostasis

Not reported

Inclusions

Not reported

Motor Behaviours

Not reported

Response to dopaminergic treatment

Not reported

Non-motor Behaviours

Not reported

Electrophysiology

Not reported