The sequence coding for the mouse Parkin protein is expressed under the control of the rat neuron-specific enolase promoter (NSE).
Endogenous Parkin: Yes
Corresponding human genotype: Park2 was the first gene associated with autosomal recessive Parkinson’s disease (PD).
Targeted gene: Parkin
References: Bian 2012
Overexpression of the mouse Parkin protein is observed in different brain regions, including the striatum, substantia nigra, cortex, hippocampus and cerebellum (0.3-0.8 fold increase in transgenic compared to wild type mice).
2-3 and 11-13 months: No visible degeneration can be observed in the SN or striatum of the transgenic animals. However, Parkin overexpressing mice are less sensitive to treatment with the MPTP toxin.
2-3 and Striatal levels of alpha-synuclein are comparable in young transgenic and wild type mice.
11-13 months: Older transgenic mice display a lower level of striatal alpha-synuclein when compared to age-matched wild type mice.
Response to dopaminergic treatment